Apoptosis Event in Hela Cells Treated with Cisplatin, Gallic Acid and Combination of Both

Adverse effect of conventional chemotherapeutic agent using single anticancer drugs had led to application combination therapy in cancer treatments. Combination polyphenolic compounds and was studied shown promising outcomes vitro. In treatment, mode cell death through apoptosis is one the targeted approaches many preclinical drug development studies has been identified as a key mechanism chemotherapy-induced death. 
 Hence, this study conducted evaluate event HeLa cells treated with gallic acid (GA) combined cisplatin (CIS). were for 24,48 72 hours CIS GA at IC50 (Table 1) concentrations, (CIS– GA) chosen based on previous (66.73 percent inhibition [1]. To compare effects individually in-combination cells, flow cytometry assay carried out. According findings, during every incubation period, CIS-GA treatment group consistently showed highest level antiproliferative activity followed by group. response time-dependent, an increase apoptotic time (Figure 1). At hours, significant improvement (74.6%), improvements also after 24 48 (56 60.2 percent).
 Taken together, these findings suggest that enhances magnitude cells. Additionally, have positive implications use approach treatment.